Figure 4
From: Isolation of high-purity myenteric plexus from adult human and mouse gastrointestinal tract
Immunofluorescence stainings of neurospheres differentiated on ECM-coated coverslips for 6 days.
Proliferation was revealed using BrdU-incorporation (blue). The phenotype of the cells was visualized via the expression of Nestin (green) and the pan-neuronal marker PGP9.5 (red) for neurons (A) and S100b for glial cells (B). The proliferation was detected in the S100b+ nestin-expressing glial population. Individual stainings are depicted for the stomach (St) only. All other segments are shown as merged images. Magnification 200×. Scale bar: 100 μm.
