Figure 1 | Scientific Reports

Figure 1

From: Breeding scheme and maternal small RNAs affect the efficiency of transgenerational inheritance of a paramutation in mice

Figure 1

A paramutation displaying the “white-tail-tip” phenotype induced by an insertional mutation in Kit locus in mice.

(A) Generation of a knock-in allele, which contains copGFP, HPRT and PGK gene cassettes, located immediately downstream of the start codon in exon 1 of Kit gene. (B) Kit+/copGFP mice display white tail tips (WTTs), white bellies and white paws (left panel), whereas a proportion of the wild-type F1 offspring derived from heterozygous parents show white tail tips (middle panel). Some wild-type129Sv/Ev mice in our colony display white tail tips (right panel). Scale bar = 1 cm. (C) Various types of white tail tip (WTT) phenotype in both the general wild-type C57BL/6J mouse colonies and the Kit paramutant families. (D) Penetrance of the WTT phenotype in F1 offspring of heterozygous parents. (E) Penetrance of the WTT phenotype in F1 offspring from heterozygous mothers. (F) Penetrance of the WTT phenotype in F1 offspring from heterozygous fathers. “n” denotes the total number of F1 offspring observed in each of the three mating schemes (D–F).

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