Figure 2
From: Fibronectin localization and fibrillization are affected by the presence of serum in culture media
FN staining on HDFs.
“General” FN was stained via FN-15 antibody (A and B) and ED-A FN via IST-9 (C and D, 10 ng/mL TGF-β1) and both exhibited a fibrillar organization without serum (A and C) and a predominantly non-fibrillar one under 10% FBS. Oval insets show magnified views of the interconnection between fibronectin (red) and actin (green) in the absence of serum, while they appear mutually exclusive in its presence. Panels E and F represent the images in A and D in inverted colours (cyan for FN, magenta for actin) for a clearer appreciation of spatial relation of fibronectin with actin. Panels G to I summarize the different morphology of endogenous FN with serum (spotted, G) or without serum (fibrillar, H) and actin (fibrillar but with a different pattern); images G to I were obtained by first inverting the colours of DAPI+actin/FN-stained cells and then converting them to grayscale.
