Figure 5
From: Fibronectin localization and fibrillization are affected by the presence of serum in culture media
Confocal microscopy of ED-A FN (red) imaged with the IST-9 antibody.
The non-fibrillar form of FN appears to be present dorsally and localized between neighboring actin fibres (green); within the resolution of the technique, there is no apparent difference in vertical position between actin and FN. It should be noted that the low intensity ventral red fluorescence is actually due to dorsally localized components that cannot be completely separated due to a cell thickness (<100 nm, see AFM pictures in Figure 4, bottom) of the same order of magnitude of a confocal voxel. On the contrary, the fibrillar form recorded in serum-free medium was clearly positioned ventrally, in between cell body and substrate.
