Figure 6
From: Fibronectin localization and fibrillization are affected by the presence of serum in culture media
Localization of gelatinolytic sites (green fluorescence) and actin (red fluorescence) for HDFs cultured under serum-free conditions (top) or in 10% FBS (bottom) and with (right) or without (left) 10 ng/mL TGF-β1.
DQ™ gelatin fluorescein groups are quenched in the macromolecule prior to degradation due to its high degree functionalization, whereas they fluoresce upon enzymatic cleavage with an intensity proportional to the extent of degradation. The oval insets show magnified portions of the pictures in inverted colours for an easier appreciation of the localization of DQ™ gelatin fluorescence predominantly between actin fibres.
