Figure 8
From: Fibronectin localization and fibrillization are affected by the presence of serum in culture media
Fluorescence images for HDFs cultured in 10% FBS without TGF-β1.
(A): ED-A FN (red) and degraded gelatin (green) showed an almost perfect colocalization; please note, although in this part of the study we have focused on ED-A FN, FN and ED-A FN share the same localization pattern (Figure 2). (B): α-SMA was substantially absent without TGF-β1, but in myofibroblasts it mapped the localization of F-actin and thus showed a virtually complete exclusion with ED-A FN. (C): Evidence of mutual exclusion between actin and DQ™ gelatin, as shown also in Figure 5. (D): MMP-3 (green) showed a predominantly fibrillar distribution that colocalized with that of actin (red), although some protease also accumulated in the perinuclear area. The separate green and red channels for all pictures are presented in Supplementary Information, Figure S7, right. Please note that 10 ng/mL TGF-β1 provided indistinguishable results, see Supplementary Information, Figure S8.
