Figure 3

Migration was reversed after co-transfected with miR-206 inhibitor and SDPR siRNA.

(A): Sequence alignment of human miR-206 with 3' UTR of SDPR. Bottom: mutations in the 3'-UTR of SDPR in order to create the mutant luciferase reporter construct. (B): Cells were transfected with miR-206 and control, renilla luciferase vector pRL-SV40 and SDPR3'UTR luciferase reporters for 48 h. Both firefly and Renilla luciferase activities are measured in the same sample. Firefly luciferase signals were normalized with Renilla luciferase signals. All tests were performed in triplicate and presented as mean ± SE. (C): The cell biological changes in migration and proliferation were reversed after co-transfected miR-206 inhibitor and SDPR siRNA. Cells were divided into four groups in two cell lines, respectively. The first group was transfected with control, followed by miR-206 inhibitor, SDPR siRNA and the last one that co-transfected with miR-206 and SDPR siRNA. We compared the co-transfected group with others and found migration and proliferation were partly reversed after co-transfected with miR-206 inhibitor and SDPR siRNA when compared with the cells transfected with miR-206 inhibitor and were much more restored after co-transfected with miR-206 inhibitor and SDPR siRNA. Data were presented as mean ± SEM from three separate experiments performed in triplicates and were analyzed by double-sided Student's t-test. (* indicates P < 0.05).