Figure 3

Phylogenetic analysis, expression profiles and abundances of pheromone biosynthesis-related transcripts in Helicoverpa armigera and Helicoverpa assulta.
The phylogenetic tree was constructed in MEGA6.0 using the neighbour-joining method. Bootstrap values >50% (1000 replicates) are indicated at the nodes. Transcripts that were too short for phylogenetic analysis are listed under the respective trees. Expression levels of acetyl-CoA carboxylase, aldo-Ketose Reductase, desaturase and fatty acyl-CoA related transcripts were determined in female bodies without pheromone glands (B1) and PGs by semi-quantitative RT-PCR. Transcripts from H. armigera are labelled in red and H. assulta in blue. Transcript expression abundance is indicated as RPKM values. The PG-biased ACC, ALR, FAS, and DESs are labelled with pentagrams in the phylogenetic tree. The gene for GTP-binding protein was used as the positive control.