Figure 3
From: Circulating Peptidome to Indicate the Tumor-resident Proteolysis
Comparison of natural C3f fragments and the cleavage site analysis after incubation with MMP-9, serum and cell culture media indicate tumor protease specificity.
(A) Observed cleavage sites on C3f. Arrows show the observed cleavage sites. Red arrows show the cleavage sites of interest. The series of b- and y-ions in MALDI-TOF/TOF MS spectrum confirmed the amino acid sequence of these two peaks as (B) 6xHis-SSATTFRL (m/z = 1704.8) and (C) LWENGNLLR-6xHis (m/z = 1936.9). Insets show the zoomed-in view of the MS-MS spectra.
