Figure 4
From: Circulating Peptidome to Indicate the Tumor-resident Proteolysis
MMP-9 expression in cell culture media correlates with the extent of cleavage of the L1311 – L1312 peptide bond in C3f.
(A) Immunoblotting using equivalent sample volumes of conditioned media from cells transfected with control siRNA, EphA2 siRNA, or MMP-9 siRNA. To confirm MMP-9 expression levels. All gel and blots run under same condition, the full image as shown in Figure S4. (B) The relative peak intensities of two specific fragments, 6xHis-SSATTFRL (m/z = 1704.8) and (C) LWENGNLLR-6xHis (m/z = 1936.9), generated by incubating synthetic C3f with culture media under the same conditions indicated in (A). **, p < 0.01.
