Table 1 CDS coverage of each platform

From: A combination of targeted enrichment methodologies for whole-exome sequencing reveals novel pathogenic mutations

Platform

On-target region size*

CDS coverage predicted by probe design

Read bases of raw NGS data

Mean depth of CDS region [median]

CDS coverage of depth ≥10 called data

CDS coverage of depth ≥15 called data

Agilent SureSelect V4†

87.49 Mb

93.88%

6.84 Gb

79.15 [61.78]

90.57%

87.56%

Agilent SureSelect V5†

89.48 Mb

96.53%

6.38 Gb

73.10 [65.02]

93.85%

91.90%

NimbleGen SeqCap ez Human Library v2‡

81.58 Mb

96.08%

18.68 Gb

199.07 [172.00]

93.54%

92.98%

Illumina TruSeq‡

100.33 Mb

94.28%

11.40 Gb

61.72 [60.00]

90.23%

88.63%

  1. *Within 100 bp upstream and downstream of the capture targets;
  2. †SureSelect V4 and V5 data indicate average of 38 and 104 samples of our experiments, respectively;
  3. ‡the original data were obtained and have been deposited by Clark et al.9 and re-analyzed them with our analysis pipeline; More details are shown in Supplementary Table S1.
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