Figure 2
From: Titration of biologically active amyloid–β seeds in a transgenic mouse model of Alzheimer's disease
Characterization of the inoculum.
10% w/v homogenates were prepared from four 18–20 months old tg2576 animals and materials were pooled into a single stock. These brains carried large amounts of Aβ aggregates (A) which were also reactive to ThS (B). Pictures in (A) and (B) depict hippocampal and cortical regions of two different animals, respectively. Bars in both cases represent 100 μm. (C) The pooled tissue homogenate was subjected to a serial fractionation procedure (see Methods section) and the amount of aqueous insoluble Aβ42 or the levels of the total forms of insoluble Aβ were measured and compared to brains from young transgenic mice (9 months old) displaying little aggregation. Measurements were done in duplicate and data shown as average ± standard error of the mean.
