Figure 2
Quantitative detection of miRNA UR by miRFLP assay.
(a) Plot view of fluorescence peaks in panel 1, 2, 3 and 4 represented the measurements of 0, 46, 11111 and 33333 copies of UR containing equimolar of m1: miR-9-5p, m2: miR-122-5p, m3: miR-192-5p, m4: miR-92a-3p and m5: miR-451a. The std 1, std 2 and std 3 were annotated for peaks derived from iRCSs, which formed a RNA copy scale for each test. The peaks of 117.6 nt were designated for RNA spike-ins. The numbers in yellow boxes were lengths (upper, nt) and fluorescence unit (lower, FU) of DNA fragments. (b) The detected miRNA relative fluorescence units (RFU) were plotted against their corresponding reference miRNA copy numbers used. UR miRNA in amounts of 46 to 250000 copies were tested as standard curves. The standard curves for miRNA copy number conversion were fit with Power regression. (c) The dynamic detection ranges with regression coefficient estimates for assay accuracy are shown for each miRNA. The data used to construct these graphs are listed in Supplementary Tables 2 and 3.
