Table 1 The measurement of hsa-miR-92a and hsa-miR-92b from purified RNA samples
Fluorescence Unit | RFU | miRNA copy number/ng RNA | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
RNA sample, amount | iRSC #4 | iRSC #5 | iRSC #6 | miR-92b | miR-92a | miR-92b | miR-92a | miR-92b | C.V | miR-92a | C.V |
A549 RNA, 3.12 ng | 27418 | 682 | 112 | 960 | 13251 | 5635 | 151696 | 7,110 | 12.61% | 394,996 | 6.35% |
31659 | 690 | 128 | 1246 | 15984 | 6934 | 166186 | |||||
32118 | 662 | 133 | 1103 | 15867 | 5929 | 164125 | |||||
A549 RNA, 0.312 ng | 32073 | 689 | 168 | 370 | 4029 | 1273 | 27763 | 8,681 | 35.34% | 345,449 | 25.50% |
27276 | 405 | 143 | 159 | 2313 | 668 | 19071 | |||||
30185 | 496 | 119 | 212 | 3316 | 963 | 27564 | |||||
A549 RNA, 0.0312 ng | 31859 | 928 | 206 | 68 | 1149 | 93 | 4306 | 8,248 | 24.83% | 307,042 | 86.27% |
31378 | 1880 | 241 | 146 | 372 | 145 | 553 | |||||
31741 | 1087 | 186 | 87 | 1507 | 131 | 6093 | |||||
Hela RNA, 0.222 ng | 31356 | 1297 | 264 | 1487 | 7360 | 4567 | 46128 | 54,097 | 27.03% | 747,141 | 40.34% |
7536 | 198 | 39 | 184 | 946 | 3058 | 25880 | |||||
31718 | 804 | 162 | 776 | 4509 | 3144 | 30931 | |||||
H1299 RNA, 0.250 ng | 31569 | 892 | 141 | 300 | 3488 | 963 | 22175 | 7,195 | 56.09% | 509,963 | 24.15% |
31825 | 1209 | 272 | 221 | 5702 | 295 | 32215 | |||||
31733 | 1004 | 199 | 322 | 4895 | 752 | 30252 | |||||