Figure 2

Morphologic changes of RPE caused by the r15 mutation.

Rhodamine-phalloidin (for F-actin, red) and Dapi (for nuclei, blue) double labeling of whole mount RPEs from 4-week old wild-type (a) and r15 homozygous mutant (b) mice. Electron micrographs of RPEs from 5-month old wild-type (c) and r15 homozygous mutant retinas (d); white asterisks indicate apical microvilli and black asterisks indicate basal infoldings near Bruch's membrane (BM); note the absence of distinct basal infoldings and apical microvilli in the mutant retina (d). Scale bars for panels a and b, 20 μm; scale bars for panels c and d, 1 μm.