Figure 6

NHE8 is important for the polarity of ezrin expression in RPE cells.

Immunostaining of ezrin proteins (green, a marker for the apical side of RPE) and F-actin (red) in wild-type (a) and NHE8-/- retinal sections (b) at the age of postnatal day 7 (P7) show very similar ezrin distribution in the apical RPE as well as some lateral sides. At P14, ezrin distribution is mostly restricted to the apical side of RPE in the wild-type (c), while some extended ezrin expression in the lateral sides towards the basal sides is observed in the NHE8-/- retina (d, white arrows). Panels e–g show the immunodistribution of ezrin proteins in the wild-type (e), r15 mutant (f) and NHE8-/- (g) retinas of mice at the age of 4 weeks. White arrows indicate mislocalized ezrin at basal and lateral sides of r15 and NHE8-/- RPEs. Scale bar, 10 μm.