Table 1 PDZ variants selected by phage display towards the C-terminal domain of HPV18 E6 together with their binding rate constants and affinities for Lipo-E618-C
From: Design of a PDZbody, a bivalent binder of the E6 protein from human papillomavirus
PDZ variant | Mutations | koff (s−1) | kon (µM−1s−1) | Kd (μM) |
|---|---|---|---|---|
Pseudo wild type | (9)1 | 3.3 ± 0.012 | 6.2 ± 0.052 | 0.54 ± 0.012 |
PDZϕ1 | E385R (3) | 7.5 ± 0.09 | 8.2 ± 0.1 | 0.92 ± 0.02 |
PDZϕ2 | E385H, L391F, K392Q (1) | 1.5 ± 0.01 | 5.9 ± 0.08 | 0.26 ± 0.01 |
PDZϕ3 | L391F, K392A (1) | 0.75 ± 0.003 | 4.6 ± 0.03 | 0.16 ± 0.01 |
PDZϕ4 | E385H, K392R (4) | 5.1 ± 0.05 | 8.5 ± 0.1 | 0.60 ± 0.02 |
PDZϕ5 | E385H, K392T (1) | 3.8 ± 0.03 | 4.9 ± 0.05 | 0.78 ± 0.01 |
PDZϕ6 | E385K, K392Q (1) | 10.2 ± 0.3 | 4.0 ± 0.08 | 2.5 ± 0.03 |
PDZϕ7 | E385K, K392R (1) | 5.5 ± 0.06 | 8.9 ± 0.13 | 0.61 ± 0.02 |
PDZϕ8 | E385T (2) | 3.8 ± 0.04 | 5.8 ± 0.06 | 0.66 ± 0.01 |
PDZϕ9 | L391F, K392M (1) | 0.55 ± 0.002 | 5.9 ± 0.04 | 0.093 ± 0.008 |
