Figure 1
Characterization of atherosclerotic lesions in ApoE−/− mice.
(a) HE staining of aortic root sections showing the atherosclerotic lesions in ApoE−/− mice treated with or without a 12-week high-fat diet (HFD). Scale bar indicates 600 μm. (b) Quantification of the lesion area per section in the normal-diet and HFD groups. n = 3–4 mice in each group. (c) Oil red O staining of aortic root sections showing the lipid deposition in atherosclerotic lesions. Scale bar indicates 600 μm. (d) Quantification of lipid content. n = 3–4 mice in each group. (e) Representative images of TUNEL staining showing apoptotic cells (stained in green) in the endothelium. The nuclei were stained blue with DAPI. CD31 (stained in red) was used as an endothelial marker. Scale bar indicates 50 μm. The data are presented as the mean ± S.E.M., *p < 0.05.
