Figure 5
Repression of TRPC6 expression by miR-26a in HAECs.
(a) Effect of miR-26a on the protein level of TRPC6. +miR-26a inhibitor indicates the co-application of miR-26a mimics and miR-26a inhibitor. NC indicates miRNA negative control. n = 5 batches of cells. Cropped blots are shown. Full-length blots are presented in Supplementary Fig. S3 online. (b) Effect of ox-LDL on the protein level of TRPC6. n = 5 batches of cells. Cropped blots are shown. Full-length blots are presented in Supplementary Fig. S4 online. (c) The ability of miR-26a to repress ox-LDL-induced TRPC6 expression. +miR-26a indicates the co-application of miR-26a mimics and ox-LDL (50 μg/ml). +NC indicates the co-application of miRNA negative control and ox-LDL (50 μg/ml). n = 5 batches of cells. Cropped blots are shown. Full-length blots are presented in Supplementary Fig. S5 online. (d) Frozen sections of aortic root were stained for TRPC6 (green) and VE-cadherin (red). The nuclei were stained blue with DAPI. Scale bar indicates 50 μm. Arrows indicate differential TRPC6 expression. TRPC6 expression was quantified in vascular ECs. n = 5 mice in each group. (e and f) Representative images of immunofluorescence staining for TRPC6 in HAECs. The nuclei were stained blue with DAPI. Scale bar indicates 50 μm. The data are presented as the mean ± S.E.M., *p < 0.05.
