Figure 6
TRPC6 overexpression inhibits the anti-apoptotic effect of miR-26a in HAECs.
(a) Protein levels of TRPC6 detected by Western blot. +miR-26a indicates co-application of miR-26a mimics and ox-LDL (50 μg/ml). +miR-26a+TRPC6 indicates the co-application of miR-26a mimics, ox-LDL (50 μg/ml) and TRPC6 plasmids. +miR-26a+Empty indicates the co-application of miR-26a mimics, ox-LDL (50 μg/ml) and empty plasmids. n = 5 batches of cells. Cropped blots are shown. Full-length blots are presented in Supplementary Fig. S6 online. (b) Representative images of immunofluorescence staining for TRPC6 in HAECs. The nuclei were stained in blue with DAPI. Scale bar indicates 100 μm. (c) MTT assay. n = 4 batches of cells. (d and e) TUNEL staining and the averaged data of apoptotic (TUNEL-positive) cell ratio. Scale bar indicates 100 μm. n = 5 batches of cells. The data are presented as the mean ± S.E.M., *p < 0.05.
