Figure 2
From: Altered autophagic flux enhances inflammatory responses during inflammation-induced preterm labor
LC3B protein is increased and a2V mRNA is decreased in inflammation-induced preterm labor.
Panels A and C show western blots of LC3B-I, LC3B-II and GAPDH in uterus and placenta recovered from preterm labor groups and controls with corresponding densitometric analysis (LC3B-II/GAPDH) in panels B and D. LC3B-I densitometric analysis shows a similar pattern (data not shown). N = 4–5 each group. Panels E and F show LC3B and panels G and H show a2V mRNA expression in uterus and placenta recovered from preterm labor groups and controls. N = 6–11 each group. PBS, LPS and PGN+poly(I:C): intrauterine injections on day 14.5; MIF-C: subcutaneous DMSO control, MIF: subcutaneous mifepristone in DMSO on day 14.5. Error bars = ±SEM. *P ≤ 0.05, **P ≤ 0.01 Significant difference vs. respective control. Full-length blots are presented in Supplementary Figure 12.
