Figure 6
From: Altered autophagic flux enhances inflammatory responses during inflammation-induced preterm labor
NF-κB p65 activation is increased in inflammation-induced preterm labor.
(A) Distribution of LC3B, NF-κB p65 and TNF (brown) in serial sections of uterus recovered from PBS, PGN+poly(I:C) and LPS-treated animals. Six sections per animal were analyzed. Original magnification: 400X. (B) Total and phosphorylated NF-κB p65 by ELISA (control group set to 1). N = 4–5 each group. (C) mRNA expression of IL-1β and TNF in uterus by RT-PCR. N = 6–9 each group. PBS, LPS and PGN+poly(I:C): intrauterine injections on day 14.5; MIF-C: subcutaneous DMSO control, MIF: subcutaneous mifepristone in DMSO on day 14.5. Error bars = ±SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 Significant difference vs. respective control.
