Figure 7
From: Altered autophagic flux enhances inflammatory responses during inflammation-induced preterm labor
Blockade of a2V enhances baseline and LPS- and PGN+poly(I:C)-induced activation of NF-κB p65 in the RAW 264.7 mouse macrophage cell line.
RAW 264.7 cells were treated for 30 min with PBS, PGN+poly(I:C) or LPS, followed by up to 3 h incubation with IgG control or anti-a2V antibody. Each experiment was done three times with triplicates. Shown is the translocation of NF-κB p65 (green) from the cytoplasmic to the nuclear compartment (blue) in 2 h (A). Original magnification: 400X. (B) Fold change in the concentration of phosphorylated NF-κB p65 by ELISA after 1 h, 2 h and 3 h of antibody incubation (IgG group set to 1). Error bars = ±SEM. **P ≤ 0.01 Significant difference between PGN+poly(I:C) or LPS treated with PBS/IgG vs. treated with anti-a2V.
