Figure 1
Treatment of CORT (40 mg/kg, s.c.) for 7 days activated noradrenergic neuron in the LC and suppressed GABAergic neuron activity in the VLPO.
Representative and quantification of GAD+ and Fos+ double stained cell ratio in VLPO (b, c) as well as TH+ and Fos+ double stained cell ratio in LC (e, f) was calculated. Data are represented as mean ± S.E.M. (n = 5 ~ 6/group, *p < 0.05 and **p < 0.01 vs vehicle). Representative photographs of immunostained sections showing the cytoarchitectonic characteristics observed in the VLPO (a) and LC (d). Solid red arrows indicate double-labeled neurons; thin arrows indicate a neuron-immunopositive cell; hollow arrows indicate a Fos immunopositive cell. (CORT: Corticosterone; VLPO: Ventrolateral preoptic area; GAD: Glutamic acid decarboxylase; TH: Tyrosine hydroxylase).
