Figure 2

Treatment of CORT (40 mg/kg, s.c.) for 7 days didn't affect the neuronal activity in the Pef, TMN, PPT, DRN and LDT.

Quantification of Orexin+ and Fos+ double stained cell ratio in Pef (a′), ADA+ and Fos+ double stained cell ratio in TMN (b′), ChAT+ and Fos+ double stained cell ratio in PPT (c′) and LDT (e′), as well as TrpOH+ and Fos+ double stained cell ratio in DRN (d′) were calculated. Data are represented as mean ± S.E.M. (n = 5 ~ 6/group, *p < 0.05 and **p < 0.01 vs vehicle). Representative photographs of immunostained sections showing the cytoarchitectonic characteristics observed in the Pef (a), TMN (b), PPT (c), DRN (d) and LDT (e). Solid red arrows indicate double-labeled neurons; thin arrows indicate a neuron-immunopositive cell; hollow arrows indicate a Fos immunopositive cell. (ADA: Adenosine deaminase; ChAT: Choline acetylase; TrpOH: Tryptophan hydroxylase; Pef: perifronical area; TMN: Tuberomammillary nucleus; PPT: Pedunculopontine tegmental nucleus; DRN: Dorsal raphe nucleus; LDT: Laterodorsal tegmental nucleus).