Figure 3
Treatment of 7 days CORT (40 mg/kg, s.c.) decreased GR, but not MR protein levels in the LC.
Representative of western blots and quantification of total GR (a), cytoplasm GR (b), nucleus GR (c) and total MR (f) in the LC. (d) The distribution of GR in nucleus/cytoplasm after last injection of CORT. CORT 1D: CORT 40 mg/kg s.c. at day 7 proceeded by 6-day vehicle. (e) β-actin (actin) and histone-3 (histone) are shown as quantitative loading control for cytoplasm and nucleus respectively. Data are represented as mean ± S.E.M. (n = 6 pooled samples and repeated for 3 ~ 4 times, *p < 0.05 and **p < 0.01 vs vehicle).
