Figure 4

Microinjection of RU486 into the LC reversed CORT induced sleep disorders.

(a) The schematic representation of the implantation sites for location of guide cannula 1 mm above the LC and bright-field photomicrograph of a coronal brain section showing the injector tip (black arrow head) for microinjection (4V: fourth ventricle; CB: cerebellum). (b) Sleep parameters including total sleep time (TST), NREM sleep, light sleep time (LST), SWS, REM sleep and (c) sleep latency (SL), REM SL, light sleep ratio (LST%), SWS%, REM% in total sleep were assessed. RU100 and RU250: RU486 100 ng/side and 250 ng/side (everyday 30 min prior to CORT treatment for 7 days); CORT: 7 days treatment of CORT (40 mg/kg, s.c.). Data are expressed as mean ± S.E.M (n = 7 ~ 8/group, *p < 0.05 and **p < 0.01 vs Vehicle; # p < 0.05 and ##p < 0.01 vs CORT).