Table 2 The anatomical nomenclature and protocol in immunohistochemistry

From: Glucocorticoid receptors in the locus coeruleus mediate sleep disorders caused by repeated corticosterone treatment

Brain area

Coronal to bregma (mm)

Specific protein*

Source of primary antibody Catalog number

Primary antibody (12 h at 4°C)

Secondary antibody system Catalog number*

Develop solution and color

VLPO

−0.3 ~ −0.92

GAD

MAB-5406 Chemicon Millipore

1:1000

HRP Detection System (PV-9005)

DAB (brown)

Pef

−2.8 ~ −3.6

Orexin

PC-345 Chemicon Millipore

1:50

HRP Detection System (PV-9001)

DAB (brown)

TMN

−3.8 ~ −4.52

ADA

AB-176 Chemicon Millipore

1:3000

HRP Detection System (PV-9001)

DAB (brown)

PPT

−6.72 ~ −8.3

ChAT

MAB-305 Chemicon Millipore

1:1000

HRP Detection System (PV-9005)

DAB (brown)

DRN

−7.04 ~ −8.3

TrpOH

AbD, 9260-2505

1:1000

HRP Detection System (PV-9003)

DAB (brown)

LDT

−8.72 ~ −9.3

ChAT

MAB-305 Chemicon Millipore

1:500

HRP Detection System (PV-9005)

DAB (brown)

LC

−9.16 ~ −10.52

TH

22941, ImmunoStar Inc

1:2000

HRP Detection System (PV-9005)

DAB (brown)

all

 

c-Fos

Calbiochem, PC38T Merck

1:1000

HRP Detection System (PV-9001)

DAB-Ni (black)

  1. *GAD: Glutamic acid decarboxylase; ADA: Adenosine deaminase; ChAT: Choline acetylase; TrpOH: Tryptophan hydroxylase; TH: Tyrosine Hydroxylase.
  2. *All the secondary antibody systems were purchased from GBI, USA.
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