Figure 1

Tumor formation in the xenograft NOD/SCID mouse model in stage 1.

(a) Illustration of study design. Cancer tissues from 170 patients (including 126 GC, 43 CRC and 1 HCC) were procured and then xenografted into NOD/SCID mice after a small portion was set aside for pathological evaluation. In total, 80 cancer models were established and serially re-engrafted to maintain tumors in vivo. These models were finally shown to consist of 53 patient-derived carcinomas, 23 patient-derived lymphomas and 3 mouse-derived lymphomas. (b–d) Tumor formation in the xenograft NOD/SCID mouse model. (b) the representative morphology of GC and CRC patient-derived carcinomas formed in mice and (c) 3 representative cases for each cancer type (GC, CRC and HCC) from 23 human-derived lymphoma. All cases were identified with typical B cell type morphology, hCD45 positive, CD3-/CD20+ and were EBER positive. (d) Three morphologically similar cases of lymphoma that are hCD45 negative, finally shown to be mouse-derived lymphoma by negative amplification of human gene ALU (see Supplementary Table 1). Only 1 of the 3 cases were EBER+. Scale bars, 300 μm.