Figure 4

The molecular haplotyping, LD mapping and gene haplotype-specific trait association analysis in an embryo defective gene validating its strong association potential for SW in chickpea.
The genotyping of 12 SNPs, including one regulatory SNP (C/T) in the URR of this gene (A) among 244 association panel constituted four haplotypes (B). The four SNP haplotype marker-based genotyping information produced higher LD estimates covering the entire 9286 bp sequenced region of gene (C). A significant correlation of parental genotypes and RIL mapping individuals representing high (haplotype I: TAC) and low (haplotype II: CGT) SW haplotypes [constituted by three SNPs (shaded with yellow colour in B)] in URR of gene with their seed-specific diverse transcript expression (D) during seed development was evident. A superior favorable high SW-regulating haplotype (TAC) with decreased transcript expression was identified in the URR of gene (D).