Figure 1
From: The loss of Tm7sf gene accelerates skin papilloma formation in mice
Tm7sf2 gene drives cholesterol accumulation after TPA exposure.
WT and Tm7sf2 KO mice were subjected to a single skin topical application of TPA and sacrificed at the indicated time. The skin was removed and used to determine: (A) cholesterol levels by TLC analysis (n = 3); expression of (B) Abca1, (C) Hmgcr, (D) Ldlr, (F) SREB-2 by Real Time RT- PCR analyses. Expression of each gene was normalized to Gapdh and reported as 2−ΔΔCt. Relative mRNA level of WT untreated mice skin was assumed as 1. Results are given as mean ± S.D. (n = 4), *p < 0.05 vs. untreated WT, # p < 0.05 vs. the respective WT. (E) Whole skin lysates (pooled samples from n = 5 mice) were analyzed by western blotting with the indicated antibodies. Anti β-actin was used as loading control.
