Figure 2

Tm7sf2 gene regulates cholesterol sulfate homeostasis.

WT and Tm7sf2 KO mice were subjected to a single topical application of TPA and sacrificed at the indicated time. The skin was used to determine: (A) cholesterol sulfate levels by TLC analysis (n = 3); expression of (B) Sult2B1, (C) Tgm1 and (D) Inv by Real Time RT- PCR analyses. When cholesterol sulfate was used, mice of both genotypes were pre-treated with 820 μmol of cholesterol sulfate 10 min before the treatment with TPA for 4 h. Expression of each gene was normalized to Gapdh and reported as 2^−ΔΔCt. Relative mRNA level of WT untreated mice skin was assumed as 1. Results are given as mean ± S.D. (n = 4), *p < 0.05 vs. untreated WT, # p < 0.05 vs. the respective WT. (E) Whole skin lysates (pooled samples from n = 5 mice) were analyzed by Western Blotting with the indicated antibodies. Anti β-actin was used as loading control.