Figure 3

Tm7sf2 controls TPA-induced epidermal hyper-proliferation.

WT and Tm7sf2 KO were subjected to a single skin topical application of TPA and (A) skin architecture and epidermal thickness were analysed with H&E staining (magnification 200×), arrows indicate epidermal thickness; quantification was performed on n = 4 skin samples, each measured in at least 3 different fields; (B) levels of phosphorylated ERK1/2 by western blotting (n = 5 pooled samples). *P < 0.05 vs control mice of the respective genotype. WT and Tm7sf2 KO mice were subjected to a single topical application of TPA and sacrificed at the indicated time. The skin was used to determine the expression of (C) KGF and (D) Nur77 by Real Time PCR analyses. Expression of each gene was normalized to Gapdh and reported as 2^−ΔΔCt. Relative mRNA level of WT untreated mice skin was assumed as 1. Results are given as mean ± S.D. (n = 4), *p < 0.05 vs. untreated WT, # p < 0.05 vs. the respective WT.