Figure 3
Spatial training remodels dendritic complexity.
(a) Dendritic arbors measured by Golgi staining and reconstruction of the dendritic arbors in DG granular neurons of not trained wild type littermates (N-wt, 8 m old); not trained Tg2576 control mice (N-Tg2576, 8 m old); and the trained Tg2576 mice (T-Tg2576, 8 m old). (b, c) Sholl analyses of the numbers of dendritic points and quantification of total dendritic length in DG granular neurons. (d) Dendritic arbors shown by Golgi staining and reconstruction of the dendritic arbors in CA1 pyramidal neurons. (E–H) Sholl analyses of the numbers of dendritic crossing (e, f) and quantification of dendritic length of apical and basal dendritic arbors (g, h) of CA1 pyramidal neurons. Data were expressed as mean ± SD (n = 3 each group). One-way ANOVA followed by post hoc tests (LSD) was used. *, p < 0.05; **, p < 0.01 vs N-wt; #, p < 0.05; ##, p < 0.01 vs N-Tg576. Bar = 20 μm. (b) N-Tg2576 vs N-wt: p50 = 0.023; p75 < 0.001, p100 = 0.001, p125 = 0.001, p150 = 0.004, p175 = 0.004; T-Tg2576 vs N-Tg2576: p75 = 0.032, p100 = 0.011, p125 = 0.004, p150 = 0.014, p175 = 0.004. (c) N-Tg2576 vs N-wt: p = 0.008; T-Tg2576 vs N-Tg2576: p = 0.003. (e) N-Tg2576 vs N-wt: p75 < 0.001, p100 = 0.010, p125 = 0.006, p150 = 0.009; T-Tg2576 vs N-Tg2576: p75 = 0.015, p100 = 0.042, p125 = 0.006. (f) N-Tg2576 vs N-wt: p50 = 0.002; p75 = 0.005, p100 = 0.030; T-Tg2576 vs N-Tg2576: p50 < 0.001, p75 = 0.043, p100 = 0.013. (g) N-Tg2576 vs N-wt: p = 0.001; T-Tg2576 vs N-Tg2576: p = 0.001. (H) N-Tg2576 vs N-wt: p = 0.027; T-Tg2576 vs N-Tg2576: p < 0.001.
