Table 1 Antibodies employed in the study
Antibody | Specific | Type | WB | IH | Source |
|---|---|---|---|---|---|
pT205 | Phosphorylated tau at Thr205 | pAb | 1:1000 | SAB | |
pT231 | Phosphorylated tau at Thr231 | pAb | 1:1000 | SAB | |
pS262 | Phosphorylated tau at Ser262 | pAb | 1:1000 | SAB | |
pS396 | Phosphorylated tau at Ser396 | pAb | 1:1000 | Biosource | |
tau-5 | Total tau | pAb | 1:1000 | Millipore | |
Vamp2 | vesicle associated membrane protein 2 | pAb | 1:1000 | Millipore | |
Syp | Total synaptophysin | mAb | 1:1000 | Cell Signaling | |
PSD95 | Total post synaptic protein 95 | mAb | 1:1000 | Millipore | |
PSD93 | Total post synaptic protein 93 | mAb | 1:1000 | Abcam | |
GluA1 | Total GluA1 | pAb | 1:1000 | Millipore | |
GluN2A | Total GluN2A | pAb | 1:1000 | Millipore | |
GluN2B | Total GluN2B | pAb | 1:1000 | Millipore | |
PKAα | PKA catalytic subunit | pAb | 1:1000 | Santa Cruz | |
PKAβ | PKA regulatory subunit | pAb | 1:1000 | Santa Cruz | |
ERK1/2 | Total p44/42 mitogen-activated protein kinase | mAb | 1:1000 | Cell Signaling | |
pERK1/2 | Phosphorylated ERK | pAb | 1:1000 | Cell Signaling | |
CaMKII | Total CaMKII | pAb | 1:1000 | Cell Signaling | |
pCaMKII | phosphorylated CaMKII at Thr286 | pAb | 1:1000 | Cell Signaling | |
6E10 | Aβ1–16 | mAb | 1:1000 | Signet | |
4G8 | Aβ17–24 | mAb | 1:1000 | Signet | |
DM1A | α-tubulin | mAb | 1:1000 | Sigma |
