Figure 3
From: A Three-Pool Model Dissecting Readily Releasable Pool Replenishment at the Calyx of Held
Rapid and slow endocytosis do not recycle vesicles in a small recycling pool.
(A) Left: Sampled presynaptic calcium current (ICa, upper) and membrane capacitance (Cm, lower) induced by a 20 ms depolarisation applied at 0.5 s after a conditioning pulse of 20 ms depolarization with 0.3 mM GTPγS in place of GTP in the pipette solution. Right: Similar to Left, except that the stimulus interval is 20 s. (B) The model-predicted RRP replenishment curves with (scheme 1, black) and without endocytosis (scheme 2, red) after a 20 ms depolarisation pulse. Data measured with 0.3 mM GTPγS in the pipette solution are also plotted for comparison (circle). (C) Similar to A, but with a conditioning stimulus of 10 pulses of 20 ms depolarisation at 10 Hz. (D) Similar to B, except that the conditioning stimulus was 10 pulses of 20 ms depolarisation at 10 Hz.
