Figure 4
From: Developing the IVIG biomimetic, Hexa-Fc, for drug and vaccine applications
N-glycan profile of hexa-Fc and two different IVIg preparations.
MALDI-TOF mass spectra of permethylated N-glycans of (a) IVIg from Malawians, (b) IVIg GammaGard, (c) dimeric Fc and (d) hexa-Fc were obtained from the 50% MeCN fraction from a C18 Sep-Pak column (methods). Annotated structures are according to the Consortium for Functional Glycomics guidelines. All molecular ions are [M + Na]+. Putative structures are based on composition, tandem MS/MS and biosynthetic knowledge. Due to the presence of heterogeneous multiantennary structures with extended LacNAc repeats, the annotations are simplified throughout by using biantennary structures with the extensions listed in parentheses. Structures that show sugars outside a bracket have not been unequivocally defined. Circled in red is the sugar modeled in the MD simulations.
