Figure 2

Affects on cellular activity and intracellular metabolism due to temperature change.

(a), Assessments of proliferative activity in Huh7 cells at various culture temperatures (top) and re-warming to 37°C from each culture temperature at 24 hrs (middle) or 48 hrs (bottom). The coloured lines represent temperatures of 37°C (black), 33°C (green), 22°C (red), 10°C (purple) and 4°C (blue). (b), Measurements of albumin synthesis (upper), glucose concentration (middle) and lactate concentration (bottom) in the culture supernatant. The coloured lines represent temperatures of 37°C (black), 33°C (green), 22°C (red), 10°C (purple) and 4°C (blue). (c), Schematic representation of the intercellular metabolic pathways in primary hepatocytes at culture temperatures of 4, 10, 22, 33 and 37°C. The accumulation of each metabolite is indicated by coloured dots, which are highly expressed in the lower temperature condition (4, 10°C; blue), the hypothermic and body temperature condition (22, 33, 37°C; red). The dependence with temperature increases (green). (d), Relative levels of ATP (top left), ADP (middle left), AMP (bottom left), adenosine (top right) and uric acid (middle right) in primary hepatocytes under various culture temperatures. The coloured bars represent temperatures of 37°C (black), 33°C (green), 22°C (red), 10°C (purple) and 4°C (blue). *p < 0.05, vs. mean value of 4°C condition (#) by t-test. (e), Relative levels of adenylate energy charge (AEC; top left), glucose 6-phosphate/ribose 5-phosphate (G6P/R5P; top right), total amino acids (middle left), total non-essential amino acids (middle right), S-adenosylmethionine/S- adenosyl-L-homocysteine (SAM/SAH; bottom left) and putrescine/spermidine (bottom right). The coloured represent temperatures of 37°C (black), 33°C (green), 22°C (red), 10°C (purple) and 4°C (blue). *p < 0.05, vs. mean value of 4°C condition (#) by t-test.