Figure 3
From: Hypothermic temperature effects on organ survival and restoration
Functional replacement by cultured liver transplantation.
(a), Time course representation of perfusion culture and transplantation protocol. (b), Schematic representation of auxiliary liver transplantation method modified from our previously reported method42, the 70% partial hepatectomy and the portal vein stenosis. (c), Survival rate after transplantation using the cold-preserved liver with UW solution (dot line, n = 5), the cultured liver with erythrocytes (red line, n = 9) and the cultured liver without erythrocytes (black line, n = 7). Seven days after transplantation, a partial hepatectomy (PH) and PV stenosis (orange line) were performed. Statistical analyses were performed at 14 days. *p < 0.05, vs. cold preserved group by t-test. (d), Photographs of the hepatectomised host liver and transplanted donor liver at 0 and 7 days after PH and PV stenosis. Scale bars: 1 cm. (e), Liver weight after partial hepatectomy and PV stenosis. The dots represent the weights of the untreated recipient's liver (n = 12), host liver at 0 days (n = 5) and 7 days (n = 11), transplanted liver at 0 days (n = 6) and 7 days (n = 12). The data are shown as the median ± max. Statistical analysis was performed by a log-rank test using the statistical software Prism 6. (f), Assessments of serum markers after transplantation. These data are represented by ALT (red), AST (black), LDH (blue) in the top graph and albumin (green) and TBA (purple) in the bottom graph. (g), Histological analysis (top) and immunohistochemical analysis including Brd-U (second columns), albumin (third columns) and G6P(red; lower) of the hepatectomised liver (left and centre left) and transplanted liver (centre right and right) at 0 and 7 days after PH and PV stenosis. The G6P sections were counterstained with Hoechst 33342 (blue). BrdU positive cells (arrow), albumin positive cells (black arrowhead) and G6P positive cells (white arrowhead) were observed. Scale bars: 100 μm. (h), Assessment of the number of Brd-U positive hepatocytes (dotted bar) and total hepatocytes (dark bar) of the host and transplanted livers at 0 and 7 days after PH and PV stenosis. These data are shown as the mean ± s.e.m. (n = 20).
