Table 1 Changes in specific metabolites in primary hepatocytes due to changes in culture temperature

From: Hypothermic temperature effects on organ survival and restoration

Flux change between low and high temperature condition

 

•(red) Accumulation at 22, 33, 37°C

•(blue) Accumulation at 4, 10°C

•(green) Increase with temperature rise

Bile synthesis pathway

Glycolysis pathway

Amino acid metabolism pathway

1-Methylnicotinamide

Fructose 6-phosphate

Asp

Glycocholic acid

Glucose 6-phosphate

Glu

Taurocholic acid

 

Gln

 

Penthose phosphate pathway

Gly

Glutathione metabolism pathway

Ribose 5-phosphate

Saccharopine

5-Oxoproline

6-Phosphogluconic acid

Kynurenic acid

Glutathione

  
 

Purine metabolism pathway

Cholin metabolism pathway

Purine metabolism pathway

Adenosine

β-Ala

Adenine

AMP

Betaine

GTP

  
 

TCA cycle pathway

Lipid, fatty acid metabolism pathway

Polyamine synthesis pathway

Succinic acid

O-Acetylcarnitine

S-Adenosylmethionine

  

GABA

Urea cycle pathway

Urea cycle pathway

Spermidine

Citrulline

N-Acetylornithine

Spermine

Creatinine

Ergothioneine

 

N-Acetylglutamic acid

 

Urea cycle pathway

Uric acid

Bile synthesis pathway

Sarcosine

Histamine

Carnitine

4-Guanidinobutyric acid

 

Taurine

5'-Deoxy-5'-methylthioadenosine

Amino acid metabolism pathway

 

Tyr

 

Sugar-nucleotide metabolism pathway

Met

 

UDP-glucuronic acid

Ile

 

UDP-N-acetylglucosamine

Cysteinesulfinic acid

 

Gluconolactone

  

Glucuronic acid

Ketone metabolism

 

dTDP-glucose

Acetoacetic acid

 

Amino-sugar metabolism pathway

Nicotinic acid metabolism pathway

N-Acetylglucosamine 1-phosphate

Quinolinic acid

 

N-Acetylneuraminic acid

  

Glycolysis pathway

  

Glyceraldehyde 3-phosphate

  

Dihydroxyacetone phosphate

  

Amino acid metabolism pathway

  

Ala

  

Ser

  

His

  

Pipecolic acid

  

N,N-Dimethylglycine

  

Lipid metabolism pathway

  

Glycerol 3-phosphate

  

Pantothenic acid

  
  1. The metabolites that accumulated to high levels in primary hepatocytes are represented in the lower-temperature conditions (4, 10°C), the and the hypothermic and body temperature conditions (22, 33, 37°C) and the dependence on temperature increases. We represented the categorised metabolites by using a threshold value of 1.4-fold change or more in Groups 1 to 3.
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