Figure 2
From: Fluorescence-Activated Cell Sorting Analysis of Heterotypic Cell-in-Cell Structures
Optimizing factors affecting doublet formation.
(A, B, C) Doublet formation in HBSS containing different concentration of EDTA (A), or DNase (B), or FBS (C). Left panels show representative Giemsa images for doublet formation in the presence of different concentration of reagents as indicated. Right panels of column graphs show the quantification of doublet formation. Doublet percentage was calculated as dividing cells in doublets (> = 2 cells) by total cells quantified. More than 10 fields (20× objective lens) were quantified for each experiment. Data are mean ± SD of cells analyzed in triplicate and are representative of three independent experiments. (D) FACS analysis of double-positive cell population in the presence of different concentration of FBS. Upper panels show gating of controls. Lower left panels show FACS images of double-positive cell population (in quadrant Q2) in the presence of FBS (5%, 10% and 20%). Lower right graph shows quantification results of double-positive cell population in the presence of FBS. Data are mean ± SD of four independent experiments.
