Figure 1 | Scientific Reports

Figure 1

From: Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells

Figure 1

Cryopreservation maintained the phenotype of ASCs.

(A) The fibroblast-like morphology of fresh and cryopreserved ASCs (magnification 100×). Scale bars: 100 µm. (B) Fresh and cryopreserved ASCs highly expressed positive markers (CD 90, CD 73, CD 105, CD 44 and HLA ABC) while lacked of negative markers (CD 14, CD 19, CD 34, CD 45 and HLA DRDPDQ). Cryoprotective agent: 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS.

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