Figure 2
Effect of cryopreservation on ASCs viability and proliferation.
(A) Cell viability of ASCs preserved in 5% dimethylsulfoxide (DMSO) is similar to those preserved in standard cryomedium (10% DMSO + 90% fetal bovine serum (FBS)), while significantly higher than those preserved in 0.25 M trehalose (*p<0.05 relative to 0.25 M trehalose). (B) Fresh and cryopreserved ASCs displayed similar cell number cultured from day 1 to day 14, indicating proliferative potential of ASCs were maintained after cryopreservation. Cryoprotective agent: 1) 0.25 M trehalose; 2) 5% DMSO; 3) 10% DMSO; 4) 5% DMSO + 20% FBS; 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS.
