Figure 3
Cryopreservation maintained ASCs adipogenesis.
(A) Adipogenesis assessed by Oil Red O staining (magnification 400×) (scale bars: 100 µm.). (B) Similar adipogenic gene (PPAR-γ, FABP4 and LPL) expression level in cryopreserved ASCs compared to fresh ASCs (*p<0.05 relative to before induction). Cryoprotective agent: 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS.
