Figure 4
Cryopreservation maintained ASCs osteogenesis.
(A) Osteogenesis indicated by Alizarin Red staining (magnification 100x) (scale bars: 100 µm). (B) Cryopreserved ASCs expressed similar level of osteogenic markers (ALPL, OSC and RUNX2) compared to fresh ASCs (*p<0.05 relative to before induction). Cryoprotective agent: 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS.
