Figure 1 | Scientific Reports

Figure 1

From: A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

Figure 1

Outline of the chromosomal integration of gene(s) with multiple copies (CIGMC) system in Escherichia coli.

recA, encoding the DNA strand exchange and recombination protein, was previously deleted to prevent subsequent homologous recombination that could reduce the integrated copy number. kan, kanamycin resistance gene; R6K, narrow-host-range replicon; gfp, green fluorescent protein; FRT, flippase recombination target.

Back to article page