Table 2 Effect of the flippase recombination target (FRT) site number on CIGMC efficiency

From: A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

Integrated copy number

Number of strains

GPF-1

GPF-2

GPF-3

GPF-4

GPF-5

1

50

43

38

33

28

2

40

43

45

32

38

3

36

24

28

26

21

4

16

23

16

25

19

5

8

10

8

13

10

6

Nda

4

6

9

12

7

Nd

3

4

4

6

8

Nd

Nd

3

3

4

9

Nd

Nd

2

3

5

10

Nd

Nd

Nd

2

2

11

Nd

Nd

Nd

Nd

2

12

Nd

Nd

Nd

Nd

3

Average integrated copy number

2.28

2.59

2.81

3.29

3.75

  1. For each target strain, 150 clones were randomly selected to determine the integrated copy number of pG-2. The final concentration of pG-2 was 20 ng/μL.
  2. aNot detected.
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