Figure 4
From: Structure of Slitrk2–PTPδ complex reveals mechanisms for splicing-dependent trans-synaptic adhesion
Synaptogenic activities of Slitrk2 LRR1 mutants.
(a) Co-cultures of cortical neurons and beads conjugated with Fc or Slitrk2 LRR1 mutants fused to Fc. Slitrk2-induced presynaptic differentiation was monitored by immunostaining of the co-cultures with antibodies against Fc (red) and presynaptic active zone protein Bassoon (green). Scale bar, 5 μm. (b) Staining signal intensities for Bassoon on beads conjugated with Fc or Slitrk2 LRR1 mutants fused to Fc. All values represent as mean ± SEM. * p < 0.0001, compared to wild-type Slitrk2-LRR1-Fc. n = 24–29 beads.
