Figure 7
From: KRAS oncogene repression in colon cancer cell lines by G-quadruplex binding indolo[3,2-c]quinolines
Evaluation of the effect of IQc on cell viability using the ViaCount assay.
Cell populations were obtained by Guava ViaCount flow cytometry following 72 h incubation of HCT116 colon cancer, SW620 metastatic colon cancer and HEK293T embryonic kidney cell lines with 5-FU, TMPyP4, 2d, 3d and 3e at equitoxic (IC50 and IC65) concentrations, or DMSO (vehicle control). Results are expressed as the mean percentage (%) of viable (white), mid-apoptotic (grey) and dead (black) cells ± SEM, of at least three different experiments, for (a) IC50 and for (b) IC65 compound concentrations. a, p < 0.05; b, p < 0.01 from DMSO (vehicle control); c, p < 0.05; and d, p < 0.01 from 5-FU.
