Figure 4
From: Robo1/2 regulate follicle atresia through manipulating granulosa cell apoptosis in mice
Robo1 and Robo2 are primarilyexpressed in ovarian granulosa cells.
(A–B): The immunocytochemistry against Robo1 (A) and Robo2 (B) was performed on the vertical sections of the ovaries. (A1–A2): The high-magnification images of Robo1 expression as indicated by the dotted squares in (A). (B1–B2): The high-magnification images ofRobo2 expression as indicated by the dotted squares in (B). (C–E): The high-magnification images ofRobo1 expression in a primary follicle (C), a secondary follicle, (D) and a mature follicle (E). (F–H): The high-magnification images ofRobo2 expression in a primary follicle (F), a secondary follicle, (G) and a mature follicle (H). (I): The bar chart showing the comparison of integral optical density (IOD) for Robo1 expression in primary (n = 10), secondary (n = 5) and mature (n = 5) follicles. (J): The bar chart showing the comparison of the IOD for Robo2 expression in primary (n = 8), secondary (n = 5) and mature (n = 5) follicles. *p < 0.05 and **p < 0.01 indicate significant differences between the WT and Robo1/2+/− knockout groups. Scale bars = 200 μm in (A–B) and 20 μm in (A1–A2), (B1–B2) and (C–H). Negative control group: mouse IgG(K, L, M).
